Embryo Technology Laboratory
Modern technology for animal welfare and efficient breeding management
The Embryo Technology Laboratory is a joint core facility together with the Robert Koch Institute. It is located within the animal facility in Berlin-Marienfelde and provides state of the art technologies for modern mouse colony management like in vitro fertilization (IVF) and cryopreservation.
The following services are offered:
1) Embryotransfer. To guarantee a defined health status in the SPF breeding area, new transgenic mouse lines have to be imported via embryotransfer. Two cell embryos (generated by IVF or classical mating) are washed extensively to remove any pathogens and implanted into pseudopregnant foster mice.
2) Cryopreservation of mouse sperm and embryos. Mouse strains which are temporarily not needed can be stored as 2-cell embryos or sperm in liquid nitrogen. Frozen material is also an option for world-wide shipping of transgenic mouse lines.
3) In vitro fertilization. IVF is used to recover mouse lines from frozen sperm and is generally a very efficient method to generate mouse embryos.
Scientific facility management
Dr. Andreas Hutloff
Paolo Rosellini Tognetti
Dr. Uwe Klemm,
Max-Planck-Institut für Infektionsbiologie, Berlin
Dr. Geert Michel,
Transgene Technologien, FEM, Charité Universitätsmedizin Berlin
Max-Planck-Institut für Molekulare Zellbiologie und Genetik, Dresden
Frank Zimmermann, Biotechnologielabor, Universität Heidelberg