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Embryo Technology Laboratory

Modern technology for animal welfare and efficient breeding management

Introduction
Members
Cooperation partners

The Embryo Technology Laboratory is a joint core facility together with the Robert Koch Institute. It is located within the animal facility in Berlin-Marienfelde and provides state of the art technologies for modern mouse colony management like in vitro fertilization (IVF) and cryopreservation.

The following services are offered:

1) Embryotransfer. To guarantee a defined health status in the SPF breeding area, new transgenic mouse lines have to be imported via embryotransfer. Two cell embryos (generated by IVF or classical mating) are washed extensively to remove any pathogens and implanted into pseudopregnant foster mice.

2) Cryopreservation of mouse sperm and embryos. Mouse strains which are temporarily not needed can be stored as 2-cell embryos or sperm in liquid nitrogen. Frozen material is also an option for world-wide shipping of transgenic mouse lines.

3) In vitro fertilization. IVF is used to recover mouse lines from frozen sperm and is generally a very efficient method to generate mouse embryos.

Group Leader
    Dr. rer. nat. Andreas Hutloff
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Scientific facility management
Dr. Andreas Hutloff

Technical staff
Paolo Rosellini Tognetti

Additional assistance
Janni Breinl

Group Leader
    Dr. rer. nat. Andreas Hutloff
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Dr. Uwe Klemm,
Max-Planck-Institut für Infektionsbiologie, Berlin

Dr. Geert Michel,
Transgene Technologien, FEM, Charité Universitätsmedizin Berlin

Ronald Naumann,
Max-Planck-Institut für Molekulare Zellbiologie und Genetik, Dresden

Frank Zimmermann, Biotechnologielabor, Universität Heidelberg

Group Leader
    Dr. rer. nat. Andreas Hutloff
Continue to Introduction