Home Research Regine von Ramin Lab Molecular Rheumatology

Regine von Ramin Lab Molecular Rheumatology

In search of disease-relevant gene regulation patterns

Introduction
Members
Cooperation Partners
Selected Publications

Through the generous bequest from Mrs. Regine von Ramin and further financing by the Berlin senate-administration WiFoku, the Regine-von-Ramin Laboratory for Molecular Rheumatology (RvR-Laboratory) was founded in December of 2004 and is jointly used by research groups of the DRFZ and liaison groups of the Charité for the purposes of genome-wide gene expression analysis. Both chip-based Affymetrix® technology and Next-Generation Sequencing Technology (NGS) from Illumina® are used.

The RvR-Laboratory contains an Affymetrix-station, which is equipped with the the Scanner 3000 7G and the Fluidics FS450. For the purposes of RNA-sample quality control, a Bioanalyzer (Agilent) and Nanodrop ND-1000 spectral photometer are available in addition to standard instruments. Additionally, the aHybTM-Hybridisation station (Milenyi Biotec) allows for processing of array formats that are spotted on slides, and thereby processes like a miRNA expression profiling using the miRXplore Array (Miltenyi Biotec).

An ideal supplement to this facility is a Laser Capture Microdissection machine (LCM) from Fa. Arcturus, which allows a cutting out of morphologically and immune-histologically characterized tissue samples for molecular analysis without enzymatically digesting them. Both systems are primarily used for molecular analysis of cells at the level of their DNA, RNA and proteins. Through the integration of LCM-technology in the RvR-Laboratory, it is possible to perform global gene expression analysis on tissue samples that are morphologically and immuno-histologically identifiable.

The cooperation between the DRFZ and the Charité spin-off company “BioRetis”, allowed the establishment of a comprehensive mouse- and disease-relevant human transcriptome database. These datasets are freely available for analysis purposes (www.bioretis.com).

Next Generation Sequencing (NGS) technology was established for even more in-depth analyses, which makes it possible not only to analyse known gene sequences, but also the entire transcriptome including non-coding regions and so-called microRNAs, which consist of only very short gene sequences (usually 21 to 23 nucleotides) and above all have gene regulatory tasks. Furthermore, the NGS technology enables the generation of immune receptor repertoires of T- and B-lymphocytes by targeted sequencing, the determination of the microbiome by 16S sequencing.

Group Leader
    Dr. rer. nat. Andreas Grützkau
    Dr. Mir-Farzin Mashreghi

Keywords
Transcriptome analyses
Gene signatures
Gene chip analyses
Next Generation Sequencing

Continue to Members

Group leader
Dr. rer. nat. Andreas Grützkau
PD Dr. med. Thomas Häupl
Dr. rer. nat. Mir-Farzin Mashreghi

Technical management
Dr. Gitta Heinz
Dipl. Biotech. Katrin Lehmann

Technical assistance
Heike Hirseland

Group Leader
    Dr. rer. nat. Andreas Grützkau
    Dr. Mir-Farzin Mashreghi

Keywords
Transcriptome analyses
Gene signatures
Gene chip analyses
Next Generation Sequencing

Continue to Cooperation Partners

Dr. rer. nat. Joachim Grün, DRFZ

Dr. rer. nat. S Fillatreau, Dr. rer. nat. Chiara Romagnani, Dr. rer. nat. A Hutloff, Dr. rer. nat. K Tokoyoda, DRFZ

PD Dr. med. K Pels, PD. Dr. med. E. Schott, Dr. rer. nat. C Daridon, Prof. Dr. med. T Dörner, Dr. med. S Mercan,Dr. rer. nat. K Kappert, Charité – Universitätsmedizin Berlin

Prof. Dr. rer. nat. A Thiel, BCRT – Charité

Prof. Dr. rer. nat. F Melchers, Max-Planck-Institut für Infektionsbiologie, Berlin

PD Dr. rer. nat. U Höpken, Max Delbrück
Zentrum, Berlin-Buch

PD Dr. rer. med. H Eibel, Universitätsklinikum, Freiburg

Prof. Dr. med. B Bröker, Immunologie,
Greifswald

Dr. F Apparailly, Dr. H Yssel, INSERM, Montpellier, Frankreich

Prof. Dr. W Maslinski, Institute of Rheumatology, Warschau, Polen

Dr. rer. nat S. Wild, Miltenyi Biotech, Bergisch-Gladbach

Group Leader
    Dr. rer. nat. Andreas Grützkau
    Dr. Mir-Farzin Mashreghi

Keywords
Transcriptome analyses
Gene signatures
Gene chip analyses
Next Generation Sequencing

Continue to Selected Publications

Derer A, Böhm C, Grötsch B, Grün JR, Grützkau A, Stock M, Böhm S, Sehnert B, Gaipl U, Schett G, Hueber AJ, David JP. Rsk2 controls synovial fibroblast hyperplasia and the course of arthritis. Ann Rheum Dis. 2016 Feb;75(2):413-21.

Stervbo U, Bozzetti C, Baron U, Jürchott K, Meier S, Mälzer JN, Nienen M, Olek S, Rachwalik D, Schulz AR, Neumann A, Babel N, Grützkau A, Thiel A.Effects of aging on human leukocytes (part II): immunophenotyping of adaptive immune B and T cell subsets. Age (Dordr). 2015 Oct;37(5):93.

Stervbo U, Meier S, Mälzer JN, Baron U, Bozzetti C, Jürchott K, Nienen M, Olek S, Rachwalik D, Schulz AR, Waldner JM, Neumann A, Babel N, Grützkau A, Thiel A. Effects of aging on human leukocytes (part I): immunophenotyping of innate immune cells. Age (Dordr). 2015 Oct;37(5):92

Jürchott K, Schulz AR, Bozzetti C, Pohlmann D, Stervbo U, Warth S, Mälzer JN, Waldner J, Schweiger B, Olek S, Grützkau A, Babel N, Thiel A, Neumann AU. Highly Predictive Model for a Protective Immune Response to the A(H1N1)pdm2009 Influenza Strain after Seasonal Vaccination. PLoS One. 2016 Mar 8;11(3): e0150812.

Alissafi T, Hatzioannou A, Ioannou M, Sparwasser T, Grün JR, Grützkau A, Verginis P. De novo-induced self-antigen-specific Foxp3+ regulatory T cells impair the accumulation of inflammatory dendritic cells in draining lymph nodes. J Immunol. 2015 Jun 15;194(12):5812-24

Duroux-Richard I, Roubert C, Ammari M, Présumey J, Grün JR, Häupl T, Grützkau A, Lecellier CH, Boitez V, Codogno P, Escoubet J, Pers YM, Jorgensen C, Apparailly F. iR-125b controls monocyte adaptation to inflammation through mitochondrial metabolism and dynamics. Blood. 2016 Dec 29;128(26):3125-3136

Group Leader
    Dr. rer. nat. Andreas Grützkau
    Dr. Mir-Farzin Mashreghi

Keywords
Transcriptome analyses
Gene signatures
Gene chip analyses
Next Generation Sequencing

Continue to Introduction