{"id":19430,"date":"2025-08-29T14:15:53","date_gmt":"2025-08-29T14:15:53","guid":{"rendered":"https:\/\/www.drfz.de\/?p=19430"},"modified":"2025-08-29T14:15:54","modified_gmt":"2025-08-29T14:15:54","slug":"method-for-label-free-cell-analysis-and-sorting-validated","status":"publish","type":"post","link":"https:\/\/www.drfz.de\/en\/neuigkeiten\/method-for-label-free-cell-analysis-and-sorting-validated\/","title":{"rendered":"Method for label-free cell analysis and sorting validated"},"content":{"rendered":"\n<h2 class=\"wp-block-heading alignwide toc-item\" id=\"method-for-label-free-cell-analysis-and-sorting-validated\">Method for label-free cell analysis and sorting validated<\/h2>\n\n\n\n<p class=\"alignwide has-text-align-wide wp-block-paragraph\">In collaboration with the Physikalisch-Technische Bundesanstalt (PTB), researchers at the technology platform Flow Cytometry Core Facility (FCCF) at the DRFZ have successfully validated their newly developed method for label-free analysis and sorting of cells. The results were published in the journal Applied Optics.<br>The FCCF develops innovative technologies that characterize cells in a completely novel way. The light scattered by the cells is detected and analyzed with high throughput and exceptional accuracy. This detailed analysis allows cell types to be reliably distinguished without the use of special dyes or markers.<br>To verify the method, computer simulations and polymer particle measurements were compared. The excellent agreement between the mathematical model and the experiment confirmed the reliability of the approach.<br>The findings open up new application options and represent an important step forward in the further development of label-free flow cytometry.<\/p>\n\n\n\n  <section id=\"block_8acf97e0841f409cc8fcd7369053e164\" class=\"section links-list  alignwide \">\n    <div class=\"container container--center\">\n      \n      <div class=\"links-list__items-wrap\">\n        \n<div class=\"links-list__items links-list__items--row \">\n      <div class=\"links-list__item  launimation slideBottom\">\n      \n      <a href=\"https:\/\/doi.org\/10.1364\/AO.564989%20%20\" target=\"_self\" class=\"h6 links-list__item-title\">Original publication<\/a>\n\n              <span class=\"links-list__item-underline\">Jonas Gienger, Daniel Kage, and Toralf Kaiser, &quot;Pulse shapes of angle- and time-resolved scattering and interference signals in a flow cytometer,&quot; Appl. Opt. 64, 7154-7167 (2025)<\/span>\n      \n      <a href=\"https:\/\/doi.org\/10.1364\/AO.564989%20%20\" target=\"_self\" class=\"links-list__item-link\">\n        <span class=\"button button--arrow button--arrow-nw button--arrow-link\" ><\/span>\n      <\/a>\n    <\/div>\n  <\/div>\n      <\/div>\n    <\/div>\n  <\/section>\n\n\n\n\n  <section id=\"block_daa7b2dee2808b66dbe58cef921d6633\" class=\"section cta  alignwide\">\n    <div class=\"container container--center\">\n\n      <div class=\"cta__wrapper bg bg--surface\">\n        <div class=\"cta__content\">\n                      <h2 class=\"cta__heading launimation slideBottom\">Technology platform Flow Cytometry &amp; Cell Sorting<\/h2>\n          \n          \n          \n                      <div class=\"cta__ctas button-group\">\n              <a href=\"\/en\/arbeitsgruppen\/zentrallabor-zytometrie-und-zellsortierung\/\" class=\"cta__button button button--arrow launimation slideBottom\" title=\"To FCCF\" target=\"_self\"><span>To FCCF<\/span><\/a>            <\/div>\n                  <\/div><!-- content -->\n\n                  <div class=\"cta__image launimation fadeIn\">\n            <img decoding=\"async\" loading=\"lazy\" sizes=\"auto, \" src=\"https:\/\/www.drfz.de\/wp-content\/uploads\/2024\/12\/FCCF-150x84.jpg\" data-src=\"https:\/\/www.drfz.de\/wp-content\/uploads\/2024\/12\/FCCF-1024x576.jpg\" data-srcset=\"https:\/\/www.drfz.de\/wp-content\/uploads\/2024\/12\/FCCF.jpg 1920w, https:\/\/www.drfz.de\/wp-content\/uploads\/2024\/12\/FCCF.jpg 1600w, https:\/\/www.drfz.de\/wp-content\/uploads\/2024\/12\/FCCF-1366x768.jpg 1366w, https:\/\/www.drfz.de\/wp-content\/uploads\/2024\/12\/FCCF-1024x576.jpg 1024w, https:\/\/www.drfz.de\/wp-content\/uploads\/2024\/12\/FCCF-768x432.jpg 768w, https:\/\/www.drfz.de\/wp-content\/uploads\/2024\/12\/FCCF-550x309.jpg 550w\" class=\"\" alt=\"Foto FCCF\"  width=\"1024\"  height=\"576\">          <\/div>\n              <\/div><!-- wrapper -->\n\n    <\/div><!-- container -->\n  <\/section>\n\n\n\n\n<p class=\"alignwide has-text-align-wide wp-block-paragraph\">Figure: from <a href=\"https:\/\/doi.org\/10.1364\/AO.564989\">Original Publication<\/a>, license unter <a href=\"https:\/\/creativecommons.org\/licenses\/by\/4.0\/\">CC BY 4.0<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Method for label-free cell analysis and sorting validated In collaboration with the Physikalisch-Technische Bundesanstalt (PTB), researchers at the technology platform Flow Cytometry Core Facility (FCCF)\u2026<\/p>\n","protected":false},"author":4,"featured_media":19409,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[291],"tags":[535,536],"programme-area-tax":[183],"working-group-tax":[261],"class_list":["post-19430","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-publications","tag-kaiser-2","tag-technolgoy-platforms","programme-area-tax-technology-platforms-and-service-laboratories","working-group-tax-flow-cytometry-cell-sorting-fccf"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.4 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Method for label-free cell analysis and sorting validated - DRFZ<\/title>\n<meta name=\"description\" content=\"DRFZ and PTB have published their validated method for label-free analysis and sorting of cells in the journal Applied Optics.\" 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