{"id":9446,"date":"2025-02-12T11:47:43","date_gmt":"2025-02-12T11:47:43","guid":{"rendered":"https:\/\/www.drfz.de\/?post_type=working-group&#038;p=9446"},"modified":"2025-05-21T12:24:35","modified_gmt":"2025-05-21T12:24:35","slug":"flow-cytometry-cell-sorting-fccf-2","status":"publish","type":"working-group","link":"https:\/\/www.drfz.de\/en\/arbeitsgruppen\/flow-cytometry-cell-sorting-fccf-2\/","title":{"rendered":"Flow Cytometry &amp; Cell Sorting (FCCF)"},"content":{"rendered":"\n  <section id=\"technological-developments-and-innovations\"\n           class=\"section accordion  alignwide  toc-item\"\n           data-open=\"multiple\">\n    <div class=\"container container--center container--medium\">\n              <h2 class=\"h3 section__heading launimation slideBottom\"> Technological developments and innovations<\/h2>\n      \n      \n              <div class=\"accordion__filters\">\n          \n<div class=\"pills swiper\" data-has-aria=\"1\" data-event=\"accordionCategoryChanged\">\n  <div class=\"pills__wrap swiper-wrapper\" role=\"tablist\">\n      <\/div>\n<\/div>\n        <\/div>\n      \n              <div id=\"panel-\"\n             class=\"accordion__category active\" role=\"tabpanel\"\n             aria-labelledby=\"tab-\">\n                    <script type=\"application\/ld+json\">\n            {\n              \"@context\": \"https:\/\/schema.org\",\n              \"@type\": \"FAQPage\",\n              \"mainEntity\": [{\"@type\": \"Question\",\"name\": \"Cytometric pulse shape analysis \",\"acceptedAnswer\": {\"@type\": \"Answer\",\"text\": \"To increase the number of parameters of cells that can be detected by flow cytometry, we are investigating cytometric pulse shape analysis in collaboration with the Berlin-based company APE.  In this method, the resulting pulse shape of the scattered light from a cell is detected in an angle-dependent manner and classified by data processing. This allows the discrimination of cell properties based on the pulse shape alone, i.e. it is not necessary to label the cells with a surface marker, for example. In a further step, this method will be extended to enable cell sorting.\"}},{\"@type\": \"Question\",\"name\": \"quantiFlash(TM) \",\"acceptedAnswer\": {\"@type\": \"Answer\",\"text\": \"Also in cooperation with the Berlin-based company APE, we have developed the LED-based calibration tool for flow cytometers (quantiFlashTM). It enables us to quantify the sensitivity of flow cytometers.\"}},{\"@type\": \"Question\",\"name\": \"Multispectral Acquisition and Analysis of Flow Cytometric Data Using a Conventional Flow Cytometer\",\"acceptedAnswer\": {\"@type\": \"Answer\",\"text\": \"We introduced an experimental strategy, in which discontinuous emission spectra were acquired and analyzed by principal component analysis (PCA). In a proof-of-principle study we could show, that the simultaneous detection of up to 4 fluorochromes, which were all excited by a 488 nm Argon-laser could be resolved by the detection of a discontinuous emission spectrum. In other words we use a standard cytometer just by changing the optical filters as a multispectral cytometer. No further expensive modifications as described in the literature are required.\"}},{\"@type\": \"Question\",\"name\": \"A UV-C LED Sheath Fluid Desinfection Module for Flow Cytometric Cell Sorting\",\"acceptedAnswer\": {\"@type\": \"Answer\",\"text\": \"We have developed a flux reactor that sterilizes the sheath fluid at the point of use. The flux reactor consists of an array of 6 UV-C LEDs. Such UV-C radiation source is also used in water treatment and disinfection.  The module is currently installed in an INFLUX cell sorter and allows to reduce the number of microorganisms by up to 1000 times. The module was manufactured in cooperation with APE.\"}},{\"@type\": \"Question\",\"name\": \" Device to cool the sheath fluid on a FACS Aria\",\"acceptedAnswer\": {\"@type\": \"Answer\",\"text\": \"We developed a cooling device in order to keep the sheath fluid temperature on a FACS Aria constant. We found that the foccusing of the side-streams on a FACS Aria are temperature sensitve. Room and instrument temperature shifts of more than +\/- 5K results in a defocusing of the side-streams and the recovery of sorted cells decreased drastically. Furthermore, the sensitivity of delayed lasers decreased due to instable laser-delay.\nTo overcome this effects we hold the sheath-fluid constant at 17\u00b0C by using our device.\"}},{\"@type\": \"Question\",\"name\": \"Non invasive mixing of cells during long-term sorts \",\"acceptedAnswer\": {\"@type\": \"Answer\",\"text\": \"During long-term sorts cells often sediment at the bottom of the sample tube. In consequence, the threshold rate are instable or interupted and cells in the settlement are exposed to cytotoxin. In cooperation with Advalytix we modified their universal acoustic mixing platform \u201cSAWStation4\u201d so that we can implement it in a FACS Aria.\nIn our study we could show that the yield of sorted cells was about 5% higher compare to cells mixed by the original FACS Aria agitator. Moreover, we found less dead cells in sorted populations.\"}}]\n            }\n          <\/script>\n\n          <div class=\"accordion__content\">\n                          <div class=\"accordion-item  box box--accordion over launimation slideBottom\">\n                <button class=\"accordion-item__title\"\n                        aria-expanded=\"true\"\n                        aria-controls=\"accordion_69e93269b921a_0\"\n                        aria-label=\"Zeige den Inhalt an\">\n                    <span class=\"accordion-item__title-wrapper\">\n                      <span class=\"accordion-item__title-main h5\">Cytometric pulse shape analysis <\/span>\n                                          <\/span>\n                  <span class=\"icon-wrapper\"><\/span>\n                <\/button>\n\n                <div class=\"accordion-item__content-container\" id=\"accordion_69e93269b921a_0\">\n                  <div class=\"accordion-item__content\">\n                    \n                    <div class=\"accordion-item__content-text wysiwyg\">\n                      <p>To increase the number of parameters of cells that can be detected by flow cytometry, we are investigating cytometric pulse shape analysis in collaboration with the Berlin-based company APE.  In this method, the resulting pulse shape of the scattered light from a cell is detected in an angle-dependent manner and classified by data processing. This allows the discrimination of cell properties based on the pulse shape alone, i.e. it is not necessary to label the cells with a surface marker, for example. In a further step, this method will be extended to enable cell sorting.<\/p>\n\n                                          <\/div>\n                  <\/div>\n                <\/div>\n              <\/div>\n                          <div class=\"accordion-item  box box--accordion over launimation slideBottom\">\n                <button class=\"accordion-item__title\"\n                        aria-expanded=\"false\"\n                        aria-controls=\"accordion_69e93269b921a_1\"\n                        aria-label=\"Zeige den Inhalt an\">\n                    <span class=\"accordion-item__title-wrapper\">\n                      <span class=\"accordion-item__title-main h5\">quantiFlash(TM) <\/span>\n                                          <\/span>\n                  <span class=\"icon-wrapper\"><\/span>\n                <\/button>\n\n                <div class=\"accordion-item__content-container\" id=\"accordion_69e93269b921a_1\">\n                  <div class=\"accordion-item__content\">\n                    \n                    <div class=\"accordion-item__content-text wysiwyg\">\n                      <p>Also in cooperation with the Berlin-based company APE, we have developed the LED-based calibration tool for flow cytometers (quantiFlashTM). It enables us to quantify the sensitivity of flow cytometers.<\/p>\n\n                                          <\/div>\n                  <\/div>\n                <\/div>\n              <\/div>\n                          <div class=\"accordion-item  box box--accordion over launimation slideBottom\">\n                <button class=\"accordion-item__title\"\n                        aria-expanded=\"false\"\n                        aria-controls=\"accordion_69e93269b921a_2\"\n                        aria-label=\"Zeige den Inhalt an\">\n                    <span class=\"accordion-item__title-wrapper\">\n                      <span class=\"accordion-item__title-main h5\">Multispectral Acquisition and Analysis of Flow Cytometric Data Using a Conventional Flow Cytometer<\/span>\n                                          <\/span>\n                  <span class=\"icon-wrapper\"><\/span>\n                <\/button>\n\n                <div class=\"accordion-item__content-container\" id=\"accordion_69e93269b921a_2\">\n                  <div class=\"accordion-item__content\">\n                    \n                    <div class=\"accordion-item__content-text wysiwyg\">\n                      <p>We introduced an experimental strategy, in which discontinuous emission spectra were acquired and analyzed by principal component analysis (PCA). In a proof-of-principle study we could show, that the simultaneous detection of up to 4 fluorochromes, which were all excited by a 488 nm Argon-laser could be resolved by the detection of a discontinuous emission spectrum. In other words we use a standard cytometer just by changing the optical filters as a multispectral cytometer. No further expensive modifications as described in the literature are required.<\/p>\n\n                                          <\/div>\n                  <\/div>\n                <\/div>\n              <\/div>\n                          <div class=\"accordion-item  box box--accordion over launimation slideBottom\">\n                <button class=\"accordion-item__title\"\n                        aria-expanded=\"false\"\n                        aria-controls=\"accordion_69e93269b921a_3\"\n                        aria-label=\"Zeige den Inhalt an\">\n                    <span class=\"accordion-item__title-wrapper\">\n                      <span class=\"accordion-item__title-main h5\">A UV-C LED Sheath Fluid Desinfection Module for Flow Cytometric Cell Sorting<\/span>\n                                          <\/span>\n                  <span class=\"icon-wrapper\"><\/span>\n                <\/button>\n\n                <div class=\"accordion-item__content-container\" id=\"accordion_69e93269b921a_3\">\n                  <div class=\"accordion-item__content\">\n                    \n                    <div class=\"accordion-item__content-text wysiwyg\">\n                      <p>We have developed a flux reactor that sterilizes the sheath fluid at the point of use. The flux reactor consists of an array of 6 UV-C LEDs. Such UV-C radiation source is also used in water treatment and disinfection.  The module is currently installed in an INFLUX cell sorter and allows to reduce the number of microorganisms by up to 1000 times. The module was manufactured in cooperation with APE.<\/p>\n\n                                          <\/div>\n                  <\/div>\n                <\/div>\n              <\/div>\n                          <div class=\"accordion-item  box box--accordion over launimation slideBottom\">\n                <button class=\"accordion-item__title\"\n                        aria-expanded=\"false\"\n                        aria-controls=\"accordion_69e93269b921a_4\"\n                        aria-label=\"Zeige den Inhalt an\">\n                    <span class=\"accordion-item__title-wrapper\">\n                      <span class=\"accordion-item__title-main h5\"> Device to cool the sheath fluid on a FACS Aria<\/span>\n                                          <\/span>\n                  <span class=\"icon-wrapper\"><\/span>\n                <\/button>\n\n                <div class=\"accordion-item__content-container\" id=\"accordion_69e93269b921a_4\">\n                  <div class=\"accordion-item__content\">\n                    \n                    <div class=\"accordion-item__content-text wysiwyg\">\n                      <p>We developed a cooling device in order to keep the sheath fluid temperature on a FACS Aria constant. We found that the foccusing of the side-streams on a FACS Aria are temperature sensitve. Room and instrument temperature shifts of more than +\/- 5K results in a defocusing of the side-streams and the recovery of sorted cells decreased drastically. Furthermore, the sensitivity of delayed lasers decreased due to instable laser-delay.<br \/>\nTo overcome this effects we hold the sheath-fluid constant at 17\u00b0C by using our device.<\/p>\n\n                                          <\/div>\n                  <\/div>\n                <\/div>\n              <\/div>\n                          <div class=\"accordion-item  box box--accordion over launimation slideBottom\">\n                <button class=\"accordion-item__title\"\n                        aria-expanded=\"false\"\n                        aria-controls=\"accordion_69e93269b921a_5\"\n                        aria-label=\"Zeige den Inhalt an\">\n                    <span class=\"accordion-item__title-wrapper\">\n                      <span class=\"accordion-item__title-main h5\">Non invasive mixing of cells during long-term sorts <\/span>\n                                          <\/span>\n                  <span class=\"icon-wrapper\"><\/span>\n                <\/button>\n\n                <div class=\"accordion-item__content-container\" id=\"accordion_69e93269b921a_5\">\n                  <div class=\"accordion-item__content\">\n                    \n                    <div class=\"accordion-item__content-text wysiwyg\">\n                      <p>During long-term sorts cells often sediment at the bottom of the sample tube. In consequence, the threshold rate are instable or interupted and cells in the settlement are exposed to cytotoxin. In cooperation with Advalytix we modified their universal acoustic mixing platform \u201cSAWStation4\u201d so that we can implement it in a FACS Aria.<br \/>\nIn our study we could show that the yield of sorted cells was about 5% higher compare to cells mixed by the original FACS Aria agitator. Moreover, we found less dead cells in sorted populations.<\/p>\n\n                                          <\/div>\n                  <\/div>\n                <\/div>\n              <\/div>\n                      <\/div>\n        <\/div>\n          <\/div>\n  <\/section>\n\n\n\n\n  <section id=\"news\"\n           class=\"section related-news alignwide related-news--slider  toc-item\">\n    <div class=\"container container--center\">\n\n      <div class=\"related-news__wrap\">\n                  <div class=\"section__heading-container launimation slideBottom\">\n                          <h2 class=\"section__heading\">News<\/h2>\n                                  <\/div>\n        \n        \n        <div class=\"related-news__slider swiper swiper-pull-right\">\n                      <div class=\"slider__skip-links-container\">\n              <a id=\"before-slider-block_09c8a19e5235baa8cf3ea724594ec08d\"\n                 href=\"#after-slider-block_09c8a19e5235baa8cf3ea724594ec08d\"\n                 class=\"slider-skip-link\">Go to end of slider<\/a>\n            <\/div>\n          \n          <div class=\"swiper-wrapper grid graded-boxes\">\n                          <div class=\"swiper-slide\">\n                \n<article\n  id=\"post-19430\" class=\"teaser-post box box--min-height launimation slideBottom graded-box post-19430 post type-post status-publish format-standard has-post-thumbnail hentry category-publications tag-kaiser-2 tag-technolgoy-platforms programme-area-tax-technology-platforms-and-service-laboratories working-group-tax-flow-cytometry-cell-sorting-fccf\">\n  <div class=\"teaser-post__wrap\">\n    <div class=\"teaser-post__cats\">\n      \n<ul class=\"tag-pills tag-pills--small\" role=\"none\">\n      <li role=\"none\">\n      <span class=\"tag-pill\">\n        <span class=\"visually-hidden\">Kategorie: <\/span>\n        Publications      <\/span>\n    <\/li>\n  <\/ul>    <\/div>\n\n    <div class=\"teaser-post__body\">\n      <time class=\"date\" datetime=\"2025-08-29 14:15:53\">29.08.2025<\/time>\n      <div class=\"teaser-post__title h5\">\n        <a href=\"https:\/\/www.drfz.de\/en\/neuigkeiten\/method-for-label-free-cell-analysis-and-sorting-validated\/\" aria-describedby=\"post-19430\"\n          title=\"Gehe zum Beitrag\" rel=\"bookmark\">\n          Method for label-free cell analysis and sorting validated        <\/a>\n      <\/div>\n\n              <div class=\"teaser-post__excerpt ellipsis\">\n          Method for label-free cell analysis and sorting validated In collaboration with the Physikalisch-Technische Bundesanstalt (PTB), researchers at the technology platform Flow Cytometry Core Facility (FCCF)\u2026        <\/div>\n          <\/div>\n  <\/div>\n<\/article>              <\/div>\n                          <div class=\"swiper-slide\">\n                \n<article\n  id=\"post-8349\" class=\"teaser-post box box--min-height launimation slideBottom graded-box post-8349 post type-post status-publish format-standard has-post-thumbnail hentry category-publications tag-kaiser-2 tag-technolgoy-platforms programme-area-tax-technology-platforms-and-service-laboratories\">\n  <div class=\"teaser-post__wrap\">\n    <div class=\"teaser-post__cats\">\n      \n<ul class=\"tag-pills tag-pills--small\" role=\"none\">\n      <li role=\"none\">\n      <span class=\"tag-pill\">\n        <span class=\"visually-hidden\">Kategorie: <\/span>\n        Publications      <\/span>\n    <\/li>\n  <\/ul>    <\/div>\n\n    <div class=\"teaser-post__body\">\n      <time class=\"date\" datetime=\"2024-09-25 10:40:00\">25.09.2024<\/time>\n      <div class=\"teaser-post__title h5\">\n        <a href=\"https:\/\/www.drfz.de\/en\/neuigkeiten\/zellsortierung-ganz-ohne-farbstoffe\/\" aria-describedby=\"post-8349\"\n          title=\"Gehe zum Beitrag\" rel=\"bookmark\">\n          Cell sorting without dyes        <\/a>\n      <\/div>\n\n              <div class=\"teaser-post__excerpt ellipsis\">\n          Researchers at the DRFZ have developed an innovative method for cell sorting. Using Multi-Angle Pulse Shape (MAPS) flow cytometry, cells can be sorted based on\u2026        <\/div>\n          <\/div>\n  <\/div>\n<\/article>              <\/div>\n                          <div class=\"swiper-slide\">\n                \n<article\n  id=\"post-8442\" class=\"teaser-post box box--min-height launimation slideBottom graded-box post-8442 post type-post status-publish format-standard has-post-thumbnail hentry category-news-en tag-kaiser-2\">\n  <div class=\"teaser-post__wrap\">\n    <div class=\"teaser-post__cats\">\n      \n<ul class=\"tag-pills tag-pills--small\" role=\"none\">\n      <li role=\"none\">\n      <span class=\"tag-pill\">\n        <span class=\"visually-hidden\">Kategorie: <\/span>\n        News      <\/span>\n    <\/li>\n  <\/ul>    <\/div>\n\n    <div class=\"teaser-post__body\">\n      <time class=\"date\" datetime=\"2024-05-29 13:50:00\">29.05.2024<\/time>\n      <div class=\"teaser-post__title h5\">\n        <a href=\"https:\/\/www.drfz.de\/en\/neuigkeiten\/unterzeichnung-lizenzvertrag-drfz-und-firma-ape\/\" aria-describedby=\"post-8442\"\n          title=\"Gehe zum Beitrag\" rel=\"bookmark\">\n          Signing of license agreement between DRFZ and APE        <\/a>\n      <\/div>\n\n              <div class=\"teaser-post__excerpt ellipsis\">\n          The German Rheumatology Research Center Berlin, a Leibniz Institute (DRFZ) and the Berlin-based company Angewandte Physik und Elektronik GmbH (APE) signed a landmark license agreement\u2026        <\/div>\n          <\/div>\n  <\/div>\n<\/article>              <\/div>\n            \n          <\/div><!-- swiper-wrapper -->\n\n                      <div class=\"slider__pagination slider__pagination--contained\">\n              <button class=\"slider__button-prev\"><\/button>\n              <button class=\"slider__button-next\"><\/button>\n            <\/div>\n          \n                      <div class=\"slider__skip-links-container\">\n              <a id=\"after-slider-block_09c8a19e5235baa8cf3ea724594ec08d\"\n                 href=\"#before-slider-block_09c8a19e5235baa8cf3ea724594ec08d\"\n                 class=\"skip-link\">Go to beginning of slider<\/a>\n            <\/div>\n                  <\/div>\n\n                      <\/div>\n\n    <\/div><!-- container -->\n  <\/section>\n\n\n\n\n  <section id=\"projects\"\n           class=\"section related-projects alignwide related-projects--slider  toc-item\">\n    <div class=\"container container--center\">\n      <div class=\"related-projects__wrap\">\n                  <div class=\"section__heading-container launimation slideBottom\">\n                          <h2 class=\"section__heading\">Projects<\/h2>\n                                  <\/div>\n        \n        \n        <div class=\"related-projects__slider swiper swiper-pull-right\">\n          \n          <div class=\"swiper-wrapper grid graded-boxes\">\n            \n          <\/div><!-- swiper-wrapper -->\n\n          \n                  <\/div>\n\n\n                      <\/div>\n\n    <\/div><!-- container -->\n  <\/section>\n\n  ","protected":false},"excerpt":{"rendered":"","protected":false},"author":4,"featured_media":1862,"template":"","programme-area-tax":[183],"working-group-tag":[],"working-group-tax":[261],"class_list":["post-9446","working-group","type-working-group","status-publish","has-post-thumbnail","hentry","programme-area-tax-technology-platforms-and-service-laboratories","working-group-tax-flow-cytometry-cell-sorting-fccf"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.4 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Flow Cytometry &amp; 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