Mass cytometry

Mass cytometry permits deep cellular phenotyping in clinical immunology and basic research, with more than 50 parameters that can be measured simultaneously in a single assay. This is achieved by combining principles of flow cytometry and inductively coupled plasma mass spectrometry (ICP-MS), a technology that has been developed for trace metal analysis.

Please open to read on

Using mass cytometry, we perform biomarker discovery studies in several cohorts of patients with different chronic inflammatory diseases such as rheumatoid arthritis, SLE, and inflammatory bowel diseases. We follow the idea that the blood cytome contains important yet covered information about the patient’s condition and response to future therapy. We pursue cytomics to achieve precision medicine in the treatment of chronic inflammation and aim at developing a diagnostic  that can be used to select only effective treatments – individually for each patient.

In basic research, we use mass cytometry to capture the diversity of different cell types implicated in chronic inflammatory processes such as T and B lymphocytes, plasma cells and myeloid immune cells. Such diversity relates to e.g. states of cell differentiation and activation, migration and adhesion, apoptosis and survival. Understanding the complexity of cellular systems and provides new insight into the homeostasis and dynamics of immunity, which are of particular interest in cases of specialized lymphocytes bearing protective vs. autoreactive immune memory, and of immune cells that fuel acute inflammation such as monocytes.

To serve these aims at maximum quality, we strive for improving mass cytometry endeavours by developing and improving cell sample barcoding, expanding the measurement capacity  by generating new probes and by developing and validating accessory techniques to minimize experimental variations such as cell preservation and antibody cocktail lyophilisation.  Implementing these features helps to improve theaccuracy of measurements and the interpretation of highly complex multi-dimensional datasets by downstream computational analyses.

The DRFZ mass cytometry unit serves a variety of internal and external collaborations and is an active part of the worldwide mass cytometry community. The DRFZ spearheads the German Mass Cytometry Network that will be established in 2017.

Cooperation partners

Prof. Holden Maecker, HIMC/ Microbiology & Immunology, Stanford, USA

Dr. Norbert Jakubowski, Dr. Jochen Vogl, BAM, Berlin, Deutschland

Prof. Margitta Worm and PD Dr. Guido Heine, Charité Berlin

Prof. Andreas Krause, Dr. Julia Patermann, Immanual Hospital Berlin-Wannsee

PD Dr. Philipp Engelhard, PD Dr. Adrian Schreiber, Charité Berlin

Prof. Susann Müller, Leipzig, Helmholtz-Zentrum für Umweltforschung

Dr. Uta Kossatz, Univ. Tübingen, Deutschland

Prof. Stefan Schreiber, Univ. Klinik Schleswig-Holstein, Kiel, Deutschland

PD Dr. Thomas Häupl, Dr. Till Sörensen, Dr. Melanie Conrad, PD Dr. Adrian Schreiber, Dr. Angela Ariza de Schellenberger, Charité Berlin

Dr. Desiree Kunkel, BCRT, Berlin, Deutschland

Dr. Christian Dose, Dr. Susanne Krauthäuser, Miltenyi Biotec, Bergisch-Gladbach, Deutschland

Dr. Larissa Müller, Thermo Fisher, Bremen

Ben Pacheco, PhD, Cytodiagnostics, Burlington, ON, Canada

Fluidigm Sciences, South San Francisco, CA, USA

Spherotech Inc., Lake Forest, IL, USA

Selected publications

Baumgart, S., Peddinghaus, A., Schulte-Wrede, U., Mei, H. E. & Grutzkau, A. OMIP-034: Comprehensive immune phenotyping of human peripheral leukocytes by mass cytometry for monitoring immunomodulatory therapies. Cytometry A 91, 34-38, doi:10.1002/cyto.a.22894 (2017).

Schulz, A. R., Stanislawiak, S., Baumgart, S., Grutzkau, A. & Mei, H. E. Silver nanoparticles for the detection of cell surface antigens in mass cytometry. Cytometry A 91, 25-33, doi:10.1002/cyto.a.22904 (2017).

Mei, H. E., Leipold, M. D. & Maecker, H. T. Platinum-conjugated antibodies for application in mass cytometry. Cytometry A, doi:10.1002/cyto.a.22778 (2015).

Mei, H. E., Leipold, M. D., Schulz, A. R., Chester, C. & Maecker, H. T. Barcoding of live human peripheral blood mononuclear cells for multiplexed mass cytometry. J Immunol 194, 2022-2031, doi:10.4049/jimmunol.1402661 (2015).

Sorensen, T., Baumgart, S., Durek, P., Grutzkau, A. & Haupl, T. immunoClust–An automated analysis pipeline for the identification of immunophenotypic signatures in high-dimensional cytometric datasets. Cytometry A 87, 603-615, doi:10.1002/cyto.a.22626 (2015).

The GERman MAss cytometry NETwork (GERMANET)

project supervision – exchange of expertise – technology transfer – data analysis

The recently developed mass cytometry (CyTOF technology) captures the complexity of cellular systems at unprecedented depth, promising significant contributions in biomedical research and diagnostics. The German Rheumatism Research Center Berlin (DRFZ) initiates the foundation of a nationwide mass cytometry network, GERMANET (GERman MAss cytometry NETwork), that bundles expertise of all German mass cytometry centers, at the DRFZ, the Berlin-Brandenburg Center for Regenerative Therapies (BCRT, Charité Berlin), the Center for Regenerative Therapies of the TU Dresden (CRTD), and the University Clinics Ulm. The complexity of mass cytometry projects represents significant hurdles and risks for individual researchers, which will be minimized by interconnecting and joining forces of German mass cytometry centers along a divide-and-conquer strategy. Aims of this network are to i) boost communication between new and experienced users, technology developers, and data analysis experts, to benefit individual researchers projects, ii) the exploitation of synergies in panel design and technology development arising from the centers complementary research scope, iii) quality assurance of mass cytometric analyses and data, and their reproducibility, and iv) facilitating bioinformatic analyses. This will be achieved by i) intense project supervision in the planning, execution and analysis phase, joint regular workshops and seminars, the installation of the researcher exchange program and mutual technology transfer, ii) the introduction of modular panels and the development of new technologies, iii) establishing joint quality standards and their integration into routines, and their validation in ring-trials, and iv) installing the Bioinformatics Contact Office. Thus, GERMANET facilitates the international competitiveness of German mass cytometry.

Henrik Mei Deutsches Rheuma-Forschungszentrum Berlin (DRFZ)
Désirée Kunkel Berlin-Brandenburger Centrum für Regenerative Therapien, Berlin (BCRT)
Jan Sodenkamp Center for Translational Cancer Research, Munich (TranslaTUM)
Manfred Hönig Universitätsklinikum Ulm
Ezio Bonifacio Center for Regenerative Therapies Dresden (CRTD)

Contact: masscytometry@drfz.de

1st  German Mass Cytometry User Forum
February 1-2, 2018, Berlin - DRFZ

Stichworte
Hochdimensionale Zellvermessung
Immunmonitoriting, Cytomics
Systemimmunologie
Biomarker und Biosignaturen
Biosensoren für rheumatische Entzündung
Einzelzell-Diversität

Email: masscytometry@drfz.de 

Gruppenleiter
Dr.rer. nat. Henrik Mei
Email: mei@drfz.de
Dr. rer. nat. Andreas Grützkau

Wissenschaftler
Dr. Sabine Baumgart
Dr. Axel Ronald Schulz
Tyler Burns, PhD

Technisches Personal
Silke Stanislawiak
Heike Hirseland

Doktoranden
Marie Urbicht

Studenten
Sarah Gillert
Lisa Budsinski
Julia Schulze
Edward Rullmann

Radbruch Prof. Dr. rer. nat. Andreas Radbruch Cell Biology Triantafyllopoulou_klein Dr. Antigoni Triantafyllopoulou Innate Immunity in Rheumatic Diseases Thurley Dr. Kevin Thurley Systems Biology of inflammation Ahmed Hegazy Prof. Dr. med. Dr. rer. nat. Ahmed N. Hegazy Inflammatory Mechanisms Polansky Dr. Julia Polansky-Biskup Immuno-Epigenetics Melchers Prof. Dr. Fritz Melchers Lymphocyte Development Farzin Mashreghi Dr. Mir-Farzin Mashreghi Therapeutic Gene Regulation Kruglov Dr. rer. nat. Andrey Kruglov Chronic Inflammation Loehning Prof. Dr. Max Löhning Pitzer Lab Osteoarthritis Research Kubagawa Prof. Dr. Hiromi Kubagawa Humoral Immune Regulation Hauser Prof. Dr. med. vet. Anja Erika Hauser Immune Dynamics Tokoyoda Dr. Koji Tokoyoda Osteoimmunology Scheffold Prof. Dr. rer. nat. Alexander Scheffold Cellular Immunology Niesner Dr. rer. nat. Raluca Niesner Biophysical Analytics Strangfeld Dr. med. Anja Strangfeld Pharmacoepidemiology Minden Prof. Dr. med. Kirsten Minden Paediatric Rheumatology Listing Dr. Joachim Listing Statistics & Clinical Studies Angela_Zink_230x230 Prof. Dr. Angela Zink Health Services Research Worm Prof. Dr. med. Margitta Worm Allergology Poddubnyy Prof. Dr. Denis Poddubnyy Spondyloarthritides Riemekasten Prof. Dr. med. Gabriela Riemekasten Cell Autoimmunity Hiepe Prof. Dr. med. Falk Hiepe Autoimmunology Hamann Prof. Dr. rer. nat. Alf Hamann Experimental Rheumatology Doerner Prof. Dr. med. Thomas Dörner B Cell Memory Buttgereit Prof. Dr. med. Frank Buttgereit Glucocorticoids & Bioenergetics Romagnani Prof. Dr. Chiara Romagnani Innate Immunity Hutloff Dr. rer. nat. Andreas Hutloff Chronic Immune Reactions Fillatreau Prof. Dr. rer. nat. Simon Fillatreau Immune Regulation Baumgrass Prof. Dr. Ria Baumgrass Signal Transduction Nedospasow Prof. Dr. Sergei Nedospasov Inflammation Biology