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Mass Cytometry

Each cell is unique

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2. Mass Cytometry User Forum

Mass cytometry permits deep cellular phenotyping in clinical immunology and basic research, with more than 50 parameters that can be measured simultaneously in a single assay. This is achieved by combining principles of flow cytometry and inductively coupled plasma mass spectrometry (ICP-MS), a technology that has been developed for trace metal analysis.

Using mass cytometry, we perform biomarker discovery studies in several cohorts of patients with different chronic inflammatory diseases such as rheumatoid arthritis, SLE, and inflammatory bowel diseases. We follow the idea that the blood cytome contains important yet covered information about the patient’s condition and response to future therapy. We pursue cytomics to achieve precision medicine in the treatment of chronic inflammation and aim at developing a diagnostic that can be used to select only effective treatments – individually for each patient.

In basic research, we use mass cytometry to capture the diversity of different cell types implicated in chronic inflammatory processes such as T and B lymphocytes, plasma cells and myeloid immune cells. Such diversity relates to e.g. states of cell differentiation and activation, migration and adhesion, apoptosis and survival. Understanding the complexity of cellular systems and provides new insight into the homeostasis and dynamics of immunity, which are of particular interest in cases of specialized lymphocytes bearing protective vs. autoreactive immune memory, and of immune cells that fuel acute inflammation such as monocytes.

To serve these aims at maximum quality, we strive for improving mass cytometry endeavours by developing and improving cell sample barcoding, expanding the measurement capacity by generating new probes and by developing and validating accessory techniques to minimize experimental variations such as cell preservation and antibody cocktail lyophilisation. Implementing these features helps to improve theaccuracy of measurements and the interpretation of highly complex multi-dimensional datasets by downstream computational analyses.
The DRFZ mass cytometry unit serves a variety of internal and external collaborations and is an active part of the worldwide mass cytometry community. The DRFZ spearheads the German Mass Cytometry Network (GerMaNet) that was established in 2017.

Mass cytometry Dr.rer. nat. Henrik Mei Phone +49 (0)30 28460 - mei@drfz.de more
Immune Monitoring || Regine von Ramin Lab Molecular Rheumatology Dr. rer. nat. Andreas Grützkau Phone +49 (0)30 28460-690 gruetzkau@drfz.de more
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Gruppenleiter
Dr. rer. nat. Henrik Mei
Dr. rer. nat. Andreas Grützkau

Wissenschaftler
Dr. rer.nat. Sabine Baumgart
Dr. rer.nat. Axel Ronald Schulz

Doktoranden
Antonia Niedobitek

Bachelor/Diploma/Master
Lisa Budzinski, Sarah Gillert
Edward Rullmann,
Julia Schulze, Sarah Gillert, Christina Schäfer

Technische Assistenz
Heike Hirseland,
Anette Peddinghaus,
Silke Stanislawiak

Coordinator
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Prof. Holden Maecker, HIMC/ Microbiology & Immunology, Stanford, USA

Dr. Norbert Jakubowski, Dr. Jochen Vogl, BAM, Berlin, Deutschland

Prof. Margitta Worm and PD Dr. Guido Heine, Charité Berlin

Prof. Andreas Krause, Dr. Julia Patermann, Immanual Hospital Berlin-Wannsee

PD Dr. Philipp Engelhard, PD Dr. Adrian Schreiber, Charité Berlin

Prof. Susann Müller, Leipzig, Helmholtz-Zentrum für Umweltforschung

Dr. Uta Kossatz, Univ. Tübingen, Deutschland

Prof. Stefan Schreiber, Univ. Klinik Schleswig-Holstein, Kiel, Deutschland

PD Dr. Thomas Häupl, Dr. Till Sörensen, Dr. Melanie Conrad, PD Dr. Adrian Schreiber, Dr. Angela Ariza de Schellenberger, Charité Berlin

Dr. Desiree Kunkel, BCRT, Berlin, Deutschland

Dr. Christian Dose, Dr. Susanne Krauthäuser, Miltenyi Biotec, Bergisch-Gladbach, Deutschland

Dr. Larissa Müller, Thermo Fisher, Bremen

Ben Pacheco, PhD, Cytodiagnostics, Burlington, ON, Canada

Fluidigm Sciences, South San Francisco, CA, USA

Spherotech Inc., Lake Forest, IL, USA

Coordinator
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German Mass Cytometry User Forum

German Mass Cytometry User Forum

2nd German Mass Cytometry User Forum

www.drfz.de/cytof-forum-2019/

Coordinator
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